June 18, 2019 | Tuesday | 11AM EST
Clarification of non-settled Lenti suspension harvest can be challenging due to high level of impurities in the feed stream, physical characteristics of the vector, limited filter choices, and low recovery. A series of clarification studies led to the identification of two feasible clarification filter set up options that provide workable outcomes regarding product recovery, impurity reduction, process throughput and scalability.
Key Lessons Learned:
- Solution for clarification of non-settled Lenti suspension harvest challenges.
- Two workable clarification filter set up options were developed.
- It is feasible to clarify non-settled suspension harvest at 1000L scale or larger.
Stephen Tottey, Ph.D.
Steve Tottey has more than fifteen years of experience in chromatography and characterization. His interest is in the application of science to improve human life. He currently works at WuXi AppTec as an Associate Principal Scientist in the Process and Technology Development department. He is responsible for developing processes for the purification of viral vectors at the bench and GMP manufacturing scale.
Steve has authored multiple book chapters and thirty-eight peer-reviewed journal publications.
John Taylor is a Process Development Scientist with MilliporeSigma, based in the Philadelphia, Pennsylvania area. John has a B.S. in Biological Engineering from North Carolina State University and is earning a M.S. in Biochemical Engineering at Villanova University. At MilliporeSigma, John regularly conducts biologics filter sizing trials and engages in purification process development thought leadership with customers in the Pennsylvania, New Jersey and New York region of the United States. When he isn’t filtering, John enjoys traveling, and dancing at concerts.
Life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.