Summary
We have previously developed stable lentiviral vector (LVV) packaging and producer cell lines. The aim of the work presented here was to further test the performance of our stable cell lines.
Our cargo gene silencing system reduced SFFV-, EFS-, and EF-1α-driven cargo gene expression during production up to 95%.
LVV titre was boosted up to four-fold by cargo gene silencing.
Custom cell line development timeline has been shortened by six weeks.