The figures to the right demonstrate how reconfiguration improves infectivity.

In the first image you can see how rAAV5 made using standard plasmids (left) infects HEK293 cells less efficiently than rAAV5 made using OXGENE’s reconfigured RepCap plasmid (right).

The table just below the image provides data from a third-party evaluation of OXGENE’s AAV5 plasmids using four different HEK293 cell lines. Highest and lowest results reported. VG titrated by ddPCR, physical particles by capsid ELISA.

Our HEK293 suspension cell line has been used for producing a large number of batches of LV vectors containing various therapeutic transgenes for our customers, and our in-house testing also showed a good versatility of the cell line when producing a range of LV vectors encoding various sizes of GOIs with various sizes.

The figure to the right demonstrates LV vector production in HEK293 suspension cell line for various transgenes. All productions were performed in 24 deep-well plates. Infectious titration by qPCR measurement of integrated vector copy number in transduced HEK293T cells. N = four production replicates, with three transfection replicates per production. Error bars represent standard deviation.

Together with our standard process, we offer services of testing various promoters with clients’ GOIs therapeutic cargos to assess both LVV production titres and GOI cargo expression levels, and clients can also test the LVVs in the target cells for the relevant functions to ensure that the best promoter is selected for the LVV product.

We offer customized projects aimed to support you in the early-stage discovery and development of your program.

Below are some examples of custom projects we can undertake.

• Testing various promoters with clients’ GOIs therapeutic cargos to assess both viral vector production titres and GOI cargo expression levels
• Screening of various parameters at different scale and production modes (24-well plates, E125 flasks, AMBR15)
• Migrating from flask scale to Stirred Tank Reactor (E125, 1L, 3L and 10L STR)
• Optimizing downstream processing (chromatography, TFF, sterile filtration)
• Developing analytical methods (PCR-based detection methods, ELISA, Flow cytometry-based detection methods)
• Quality control and sequence verification such as:
  • Nanopore GridION instrument permits five parallel runs
  • P2 Solo permits use of PrometION flowcells for high fidelity applications
  • Robust methods for sequencing:
    • Plasmids
    • AAV
    • Adenovirus
    • Amplicons
    • Human cell lines
  • In-house bioinformatics pipelines for data processing and alignment to a reference
  • Bespoke AAV contamination pipeline and specialist expertise to investigate:
    • Reverse packaging (genome transfer plasmid backbone)
    • Packaging of Helper plasmid DNA
    • Packaging of RepCap plasmid DNA
    • Packaging of human cell line DNA